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Identification and Characterization of Crr1a, a Gene for Resistance to Clubroot Disease (Plasmodiophora brassicae Woronin) in Brassica rapa L.

Figure 2

Complementation test of Crr1a candidate.

A. Resistance of transgenic T2 lines (UpCc_01–24), LsUbi promoter::GUS lines (UGU_07, 08), and wild-type Col-0 to Ano-01. Means (±SD) are based on the average of 2 or 3 clubroot tests (9 plants per test). A lower mean disease index score indicates higher resistance to clubroot. B, C. Root phenotypes of (B) Col-0 inoculated with Ano-01 and (C) transgenic T2 line inoculated with Ano-01. Scale bar indicates 10 mm. D. Resistance of T3 lines to Ano-01 (A) and Wakayama-01 (W).

Figure 2

doi: https://doi.org/10.1371/journal.pone.0054745.g002