Skin Collagen Fiber Molecular Order: A Pattern of Distributional Fiber Orientation as Assessed by Optical Anisotropy and Image Analysis
Figure 5
Birefringent images with focal plane changes.
The microscope’s focal plane was sequentially altered from the upper to the lower surface (A to F) of a thick section of skin. The morphological changes observed depend on the track followed by the CBs from one focal position to the next. The large arrows in the X- and Y-axis directions represent the polarizer and analyzer PPL, respectively. Bar = 50 µm.