Sources of Variability in Platelet Accumulation on Type 1 Fibrillar Collagen in Microfluidic Flow Assays
Figure 2
Sensitivity of platelet accumulation to collagen surface density.
Type 1 fibrillar collagen was adsorbed to clean glass slides at solution concentrations of 5–1000 µg/mL. Whole blood was perfused over the collagen substrates at 300 s−1 and platelet accumulation was measured by fractional surface coverage. There was significantly lower platelet accumulation on 5 µg/mL and 10 µg/mL surfaces than on surfaces prepared from solutions of greater than 50 µg/mL. Lines with ** denotes a p<0.01 for the Mann-Whitney U-test.