Taxifolin Enhances Andrographolide-Induced Mitotic Arrest and Apoptosis in Human Prostate Cancer Cells via Spindle Assembly Checkpoint Activation
Figure 10
The combined effect of Andro and Taxi on enhancing tubulin assembly in vitro.
Tubulin was incubated at 37°C in the reaction buffer in the presence of (A) 1% DMSO (vehicle control), Andro (20, 40 or 200 µM) or 10 µM of Taxol (positive control); (B) 1% DMSO; 100 µM Taxi; 40 µM Andro; 40 µM Andro+100 µM Taxi; or 10 µM Taxol. Tubulin polymerization was monitored by measuring the optical density at 340 nm for 60 min. Data are representative of three independent experiments. (C and D) The rate of increase in OD, Vmax, of the indicated conditions was plotted. The data represent the mean ± s.d. from three independent experiments.