Taxifolin Enhances Andrographolide-Induced Mitotic Arrest and Apoptosis in Human Prostate Cancer Cells via Spindle Assembly Checkpoint Activation
Figure 8
Taxi enhances Andro-induced apoptosis in DU145 cells.
The cells were treated with (A&B) 0–40 µM of Andro for 48 h, and (C) 20 µM of Andro for 24 or 48 h in the presence or absence of 100 µM of Taxi. (A) The cells were double stained with Annexin V-FITC antibody and PI and then analyzed with flow cytometry. Mean ± s.d. (n = 4) of percent of early apoptotic cell in the presence of Taxi (100 µM) and in the absence of Taxi (Taxi−) were compared. * indicates a significant difference between the two groups, *p<0.01. (B&C) Western blot analysis of caspase-7, caspase-9, and PARP in DU145 cells. 40 µg of the cell lysates were immunoblotted with antibodies against the caspases and PARP. Data are representatives of three independent experiments.