Taxifolin Enhances Andrographolide-Induced Mitotic Arrest and Apoptosis in Human Prostate Cancer Cells via Spindle Assembly Checkpoint Activation
Figure 6
Taxifolin (Taxi) enhances the growth inhibitory effect of Andro on DU145 cells.
(A) The cells were treated with 0–40 µM of Andro and 0, 100 or 200 µM of Taxi for 48 h, and cell proliferation was determined by MTT assay. Data from four independent experiments were expressed as percentage of viability compared to the vehicle control (DMSO) with standard deviation (mean ± s.d., n = 4). * indicates significantly difference from the control (p<0.01). (B) The cells were treated with 20 µM of Andro for 48 h in the presence or absence of 100 µM of Taxi, and observed under differential interference contrast microscopy. Bar = 50 µm. (C) A comparison of the viability by MTT assay of DU145, PNT2, and Hs-27 cells after the treatment with 20 µM of Andro and 100 µM of Taxi. The experiments were performed in triplicate and the standard deviations are indicated.