Taxifolin Enhances Andrographolide-Induced Mitotic Arrest and Apoptosis in Human Prostate Cancer Cells via Spindle Assembly Checkpoint Activation
Figure 5
Time course of Andro treatment on caspases and Bcl-2 family proteins expressed in DU145 cells.
The cells were incubated with 0–80 µM of Andro for 24 or 48 h, harvested and lysed. 40 µg of the cell lysate was separated in 12% polyacrylamide gel, transferred to a nitrocellulose membrane, and immunoblotted with antibodies against (A) caspase-7, -9, and -3 and substrate PARP; and (B) Bcl-2 family proteins, including Bcl-XL, Bcl-2, and Bax. Data are representative of three independent experiments.