Stat3 Inhibitor Stattic Exhibits Potent Antitumor Activity and Induces Chemo- and Radio-Sensitivity in Nasopharyngeal Carcinoma
Figure 5
Ectopic Stat3 attenuates Stattic-induced growth inhibition and apoptosis in NPC cells.
(A) NPC cells were transfected with pcDNA, Flag-Stat3 plasmids, control siRNA (Cont-si), or Stat3 siRNA (Stat3-si) and checked by western blot for the flag and Stat3 expression. (B) NPC cells described in (A) were treated with Stattic at 0–16 µM and assayed for cell viability by the MTT assay. Cell viability was calculated by the percentage of surviving cells relative to non-treated controls. (C) CNE2 cells were transfected with pcDNA, Flag-Stat3, control siRNA (Cont-si), or Stat3 siRNA (Stat3-si) and then treated with or without Stattic at 0.1 or 0.3 µM for 48 h, followed by examination for colony formation. (D) CNE1 cells (left) and CNE2 cells (right) were transfected with pcDNA or Stat3 plasmids and then exposed to the indicated doses of Stattic for 48 h, and then apoptotic cells were measured by western blot analysis of cleaved caspase-3. (E) CNE1 cells (left) and CNE2 cells (right) were transfected with control siRNA or Stat3 siRNA and then exposed to the indicated doses of Stattic for 48 h, and then apoptotic cells were measured by western blot analysis of cleaved caspase-3. Protein levels were quantified using ImageJ software. DMSO were used as control in “0” groups.