Sulodexide Decreases Albuminuria and Regulates Matrix Protein Accumulation in C57BL/6 Mice with Streptozotocin-Induced Type I Diabetic Nephropathy
Figure 13
The effect of Gö6976, PD98059 and sulodexide on fibronectin and collagen type III synthesis and phosphorylation of signaling pathways in murine mesangial cells.
(A) Western blot analysis showing the effect of 5 mM D-glucose, 30 mM D-glucose and 30 mM mannitol in the presence or absence Gö6976 or PD98059 on fibronectin and collagen type III synthesis in murine mesangial cells after 24 h incubation (upper panel). The intensity of the bands were analyzed by densitometric scan using ImageJ (NIH), normalized to β-actin and expressed as arbitrary densitometric units (DU) (lower panels). ***P<0.001, 5 mM D-glucose vs 30 mM D-glucose, ###P<0.001, 30 mM D-glucose vs 30 mM mannitol, §§P<0.01 or §§§P<0.001, with vs without inhibitor for the same stimulation. (B) Western blot analysis showing the effect of 5 mM D-glucose, 30 mM D-glucose and 30 mM mannitol in the presence or absence of sulodexide on fibronectin and collagen type III synthesis in murine mesangial cells after 24 h incubation. (C) Western blot analysis showing the effect of 5 mM D-glucose, 30 mM D-glucose and 30 mM mannitol in the presence or absence of sulodexide on ERK PKC-α, PKC-βI or PKC-βII phosphorylation in murine mesangial cells after 24 h incubation.