Sulodexide Decreases Albuminuria and Regulates Matrix Protein Accumulation in C57BL/6 Mice with Streptozotocin-Induced Type I Diabetic Nephropathy
Figure 9
The effect of sulodexide on collagen type I gene and protein expression in renal tissue in control and DN C57BL/6 mice.
(A) Gene expression of collagen type I (Coll I) in control and DN mice treated with saline or sulodexide as determined by real-time PCR. (B) Representative images of collagen type I expression in control and DN mice at baseline and after 12 weeks treatment with saline or sulodexide are shown. Original magnification x1000. Image-based computer assisted analysis was performed to semi-quantify the amount of collagen type I in the (C) glomeruli and (D) tubulo-interstitium of control and DN mice. Results are expressed as mean+SD of data obtained from 6 mice per group. §P<0.05, §§P<0.01, §§§P<0.001, compared to baseline for the same group, #P<0.05, ###P<0.001, DN baseline vs non-diabetic baseline, ***P<0.001, DN mice vs non-diabetic mice for the same treatment, ‡P<0.05, ‡‡‡P<0.001, saline vs sulodexide treatment for the same time-point in DN mice.