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Sulodexide Decreases Albuminuria and Regulates Matrix Protein Accumulation in C57BL/6 Mice with Streptozotocin-Induced Type I Diabetic Nephropathy

Figure 6

The effect of sulodexide on phosphorylated PKC-α expression in the kidneys of control and DN C57BL/6 mice.

Representative images of (A) phosphorylated PKC-α in control and DN mice at baseline and after 12 weeks treatment with saline or sulodexide are shown. Original magnification x1000. Image-based computer assisted analysis was performed to semi-quantify the amount of phosphorylated PKC-α in the (B) glomeruli and (C) tubulo-interstitium of control and DN mice. Results are expressed as mean+SD of data obtained from 6 mice per group. §§§P<0.001, compared to baseline for the same group, ###P<0.001, DN baseline vs non-diabetic baseline, ***P<0.001, DN mice vs non-diabetic mice for the same treatment, ‡‡‡P<0.001, saline vs sulodexide treatment for the same time-point in DN mice.

Figure 6

doi: https://doi.org/10.1371/journal.pone.0054501.g006