RNA-Seq for Enrichment and Analysis of IRF5 Transcript Expression in SLE
Figure 2
Pile-up view of reads from deep sequencing of PCR-amplified IRF5 fragments.
A, Pile-up view of IRF5 sequencing reads mapped to the IRF5 genomic sequence was generated with IGV. Peak heights represent the relative frequency of mappings to the indicated regions - hills reflect a high mapping frequency of reads to a particular region and valleys indicate low mapping frequency or deletions (example shown with star); arrows point to areas of reads aligning to previously unannotated exons. Results shown are from 4 independent healthy donors and 5 independent SLE patients. B, Reads from healthy donors and SLE patients that mapped to exon 7, shown by the star in A above, and reflect true internal deletions of exon 7 are shown. Junction reads are aligned by position and labeled as to whether it was detected in a healthy or SLE sample; the exact number of reads per sample that contained the junction is shown in parentheses.