Identification of Two Evolutionarily Conserved 5' cis-Elements Involved in Regulating Spatiotemporal Expression of Nolz-1 during Mouse Embryogenesis
Figure 2
Detection of X-gal-positive signals in spinal cord and hindbrain of E9.5-E12.5 UERA–LacZ embryos.
A–J: Whole mount X-gal staining of E9.5 (A–D), E10.5 (E–G) and E12.5 (H–J) UREA-LacZ embryos. Prominent X-gal-positive signals are detected in the spinal cord (sc) of either line #22 (A, B, E, F) or #26 (C, D, G) UREA-LacZ embryos at E9.5 and E10.5. Note that, this expression is earlier and stronger in line #22 than in line #26. Interestingly, in the hindbrain (hb) region, X-gal-positive signals are detected in a selective domain of hindbrain (arrowhead) in line #26 embryos at E9.5 (C, D) and E10.5 (G). Later at E12.5, the X-gal-positive signals expend into more caudal regions of hindbrain with a pattern of longitudinal columns (arrowhead in J indicates the anterior expression border). In line #22 hindbrain, longitudinal X-gal-positive signals are detected from E10.5 at more broad regions than line #26 (E, F). H, I: By E12.5, the X-gal-positive signals are also detected in the rostral part of hindbrain (hbr, H). Asterisks in H-J indicate strong X-gal-positive signals in the paraxial mesenchyme of embryos in both lines. Notably, X-gal-positive signals are also present in the lateral plate mesoderm (lpm, H, I; arrows in G) in both lines, and in otic vesicle (ot, F and white arrow in inset at high magnification) and midbrain region (mb, I; arrows in H and inset of H) of line #22 embryos. Asterisk in F indicates a non-specific signal. fl, forelimb bud; ht, heart; hl, hindlimb bud; so, somites.