Ameloblastin in Hertwig’s Epithelial Root Sheath Regulates Tooth Root Formation and Development
Figure 5
Comparison between AMBN expressing and non expressing cells.
Full length AMBN cDNA transfected cells expressed AMBN at a high intensity whereas control cells had no AMBN mRNA. (A) and (B) Positive cell cycle regulatory factors, CDK1, CDK4 and CDK6, and negative cell cycle regulatory factors, p21Cip1 and p27Kip1, were examined by RT-PCR analysis. AMBN non expressing cells had lower expression levels of negative cell cycle regulators than those of AMBN expressing cells. (C) BrdU positive cells were counted and compared between AMBN expressing and non-expressing cells. Cell numbers in the AMBN non-expressing group was lower than those of the AMBN expressing group. Statistical analysis was performed using Student’s t-test, **; p<0.01.