Ameloblastin in Hertwig’s Epithelial Root Sheath Regulates Tooth Root Formation and Development
Figure 2
Expression of AMBN in HERS derived cells and suppression of AMBN expression by treatment with AMBN siRNA.
HERS derived cells were isolated from postnatal day 10 mice and maintained in MCDB153 medium. (A) Immuno-staining with AMBN. (B) Immuno-staining without AMBN. (C) Immuno-staining with cytokeratin 5. (D) Immuno-staining without cytokeratin 5. Scale bars: 10 µm. (E) Expression of AMBN was analyzed with total RNA from cells treated with either AMBN siRNA or control siRNA. MW; 100 bp molecular weight marker; C, treated with control siRNA; Si, treated with AMBN siRNA. Expression of AMBN was inhibited by 60% when treated with AMBN siRNA. (F) The BrdU incorporation rate of control siRNA group was higher than that of the siRNA treated group two days after siRNA treatment. Statistical analysis was performed using Student’s t-test, **; p<0.01.