Purine Nucleoside Analog - Sulfinosine Modulates Diverse Mechanisms of Cancer Progression in Multi-Drug Resistant Cancer Cell Lines
Figure 5
SF decreases the expression of mRNAs involved in tumor progression.
The expression of gst-π, hif-1α, mdr1 and vegf mRNAs in NCI-H460/R (A) and U87-TxR cells (B) after 10 µM SF treatment was evaluated relative to the internal control – gapdh and β-actin, and calculated in relation to the untreated control. Statistical significance between treated and untreated cells is presented as p<0.05 (*) and p<0.01 (**). Relative hif-1α (C), mdr1 (D) and vegf (E) mRNAs expression after 10 µM SF treatment in normoxic (absence of CoCl2) and hypoxic (presence of 50 µM CoCl2) conditions studied in NCI-H460/R cells. Statistical significance between SF treated and SF untreated cells is presented as p<0.05 (*) and p<0.01 (**).Statistical significance between CoCl2 treated and CoCl2 untreated cells is presented as p<0.01 (##) and p<0.001 (###).