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DNA Methyltransferase Inhibitor Zebularine Inhibits Human Hepatic Carcinoma Cells Proliferation and Induces Apoptosis

Figure 5

Effects of zebularine on phosphorylation of p44/42 MAPK.

The phosphorylation and expression of p44/42 MAPK after zebularine treatment for 24 h at different concentrations. After treatment, the cells were harvested and western blot analysis was performed to detect the phosphorylated and total p44/42 MAPK protein level. GAPDH was used as a loading control. Data are the means ± SEM of results from at least three independent experiments. *p<0.05, compared to 0 μM.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0054036.g005