SALL4 Expression in Gonocytes and Spermatogonial Clones of Postnatal Mouse Testes
Figure 5
Clonal organization of SALL4 expressing cells in adult mouse seminiferous tubules.
Whole mount seminiferous tubules were isolated from adult animals and subjected to immunofluorescence staining. (A–D) SALL4 was found to be expressed in As, Apr and Aal spermatogonia. Scale bars = 10 µm. (E) Quantification of the frequency of SALL4 positive As, Apr and Aal spermatogonial clones that were observed in adult mouse seminiferous tubules. Data are presented as average ± SEM from three independent experiments. The number of clones counted is indicated in parentheses beneath the graph. Total cell numbers in each clone size were calculated by multiplying the number of clones by the number of cells contained in each clone of a certain size, e.g. 254 As clones = 254 cells, 223 Apr clones = 446 cells, etc. Cell numbers are shown in parentheses.