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pAUL: A Gateway-Based Vector System for Adaptive Expression and Flexible Tagging of Proteins in Arabidopsis

Figure 2

Characterization of promoters 2x p35S CaMV, pHCF107, pHCF136, and pHCF173 fused to the GUS reporter gene.

(A) GUS staining of 5-day-old transgenic A. thaliana seedlings. (B) Histochemical localization of GUS activity in leaf sections of 3-week-old transgenic A. thaliana plants. UE, upper epidermis; PM, palisade mesophyll; SM, spongy mesophyll; LE, lower epidermis. (C) GUS activities in transgenic A. thaliana lines. In each case, 10 independent transgenic lines were tested 15 or 30 days after germination. Median values are shown as black bars and indicated at the top of each column. MU, 4-methylumbelliferone.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0053787.g002