Sox2 Is an Androgen Receptor-Repressed Gene That Promotes Castration-Resistant Prostate Cancer
Figure 9
Sox2 expression is associated with the expression of FGF5 and not established human embryonic stem cell Sox2 target genes.
A) The expression of 83 embryonic stem cell-associated genes was analyzed via quantitative real-time PCR analyses to identify Sox2-associated gene changes in LAPC-4-Sox2 cells (compared to LAPC-4-Control cells). Open bars represent genes that are previously identified Sox2-target genes in hESCs (Boyer et al. 2005). No changes in any of these 18 genes were associated with Sox2 expression in LAPC-4 cells. Rather, significant changes in expression were detected for FGF5, Kit, NR5A2, PDX1, and RUNX2 (black bars). B) Elevated expression of FGF5 in castration-resistant LAPC-4 and CWR-R1 tumors (*p<0.05; same mRNA as described in Figure 6C).