Identification and Functional Characterization of a Novel Monotreme- Specific Antibacterial Protein Expressed during Lactation
Figure 5
Identification of EchAMP protein in echidna milk.
A1, A2 and B1, B2 represent milk samples collected from two lactating echidnas at two different time points during their late-lactation phase. 70 µg protein of each sample was electrophoresed for 3 hours at 100V using a 12% SDS-Polyacrylamide gel. Bands E3 and E4 were excised from the gel, subjected to in-gel trypsin digestion and anlaysed by LTQ Orbitrap Velos. The spectra of peptides from these bands showed a significant match with the predicted EchAMP protein with high confidence levels.