Human Granuloma In Vitro Model, for TB Dormancy and Resuscitation
Figure 5
Addition of anti-TNF α monoclonal antibody caused reactivation of dormant Mtb.
(A) Auramine-O and Nile red staining of WT Mtb from IgG and anti-TNF α antibody treated granulomas. (B) Alamar Blue readouts at different time intervals when WT Mtb cells from granulomas treated with anti-TNF-α antibody or control IgG, were incubated with Alamar blue dye. (data of one representative data set has been displayed.) (C) WT Mtb cells from granulomas treated with anti-TNF-α antibody had a lower antibiotic resistance as compared to WT Mtb cells from control IgG treated granulomas. (D) Mtb cells from granulomas treated with anti-TNF α antibody catabolized fatty acids at a higher rate than Mtb from control IgG treated granuloma. P values were calculated using students t test. p<0.05 for metabolism of oleic and palmitic acid for Mtb from anti-TNF-αantibody treated granuloma compared to Mtb from IgG treated granuloma. (E) Infection of hPBMCs resulted in Apoptosis of these cells. Data are presented as mean +/− SEM from 3 experiments. P values were calculated using students t test. * p<0.05.