Expression of Foxp3 in Colorectal Cancer but Not in Treg Cells Correlates with Disease Progression in Patients with Colorectal Cancer
Figure 5
Correlation of Foxp3+ cancer cell expression with immunosuppressive cytokines IL-10+ and TGF-β+ and immunofluorescence double staining.
(A/B) Significant correlation of Foxp3+ cancer cell expression with the expression of IL-10 (A) and TGF-β (B). Regression analysis; R2, coefficient of determination. (C/D) Representative example of an immunofluorescence double staining of IL-10+ (C) and TGF-β+ (D) in Foxp3+ cancer cells (arrows). FITC green Fluoresceinisothiocyanate, Cy3 red and DAPI 4′,6-Diamidino-2- phenylindoldihydrochlorid blue – nuclear counterstaining.