Long-Term In Vivo Imaging of Fibrillar Tau in the Retina of P301S Transgenic Mice
Figure 2
FSB labels cells containing hyperphosphorylated tau in the GCL of P301S mice.
A, B In vivo SLO-examination of a P301S mouse crossed with Thy1-YFPH. A Scanning at 488 nm excitation revealed only YFP-positive cells in a unique pattern (Striking cells are numbered 1–4). B In the same retinal area, FSB-positive cells (arrows) adjacent to YFP-positive cells became apparent when excited at 450 nm. C Whole mount preparation of the in vivo examined retina (A, B). D Magnification of the boxed area in B and C. The in vivo observed FSB-positive cells (B) could be counterstained with AT8 (arrows). Axons from RGCs containing hyperphosphorylated tau appear as red background. red, AT8; white, FSB; green, YFP. Scale bars: 50 µm.