RETRACTED: Upregulation of SATB1 Is Associated with Prostate Cancer Aggressiveness and Disease Progression
Figure 5
Overexpression of SATB1 in transformed normal prostate epithelial PZ-HPV-7 cells.
PZ-HPV-7 cells were transfected with and without DNA (mock), or the SATB1-targeted vector 1 and continued in culture for 24 h. (A) SATB1, E-cadherin, MMP9 and Histone H4 protein expressions were determined in the cytosolic and nuclear fraction by Western blotting. SATB1 overexpression resulted in decrease E-cadherin expression and upregulated MMP9 expression. (B) Overexpression of SATB1 in PZ-HPV-7 cells was associated with increased invasive potential. Bars represents the mean±SE of three different assays. **P<0.001 versus control. (C) Representative PZ-HPV-7 images with and without SATB1 overexpression vector trasfection. Details are described in ‘materials and methods’ section.