Conditional Deletion of the Pten Gene in the Mouse Prostate Induces Prostatic Intraepithelial Neoplasms at Early Ages but a Slow Progression to Prostate Tumors
Figure 5
Immunohistochemistry analyses of the prostate tissues isolated from PtenloxP/loxP:Osr1-Cre.
H&E staining and immunohistochemistry were performed on prostate tissues of 4-week-old PtenloxP/loxP:Osr1-Cre mice. Adjacent prostate tissue sections from two PtenloxP/loxP:Osr1-Cre mice were analyzed with different antibodies as marked, including the AR (C, D), CK8 (G, H), E-cadherin (E, F), synaptophysin (M,N), p63 (I, J) and CK5 (K, L). A pair of adjacent slides from the prostate tissues of either PtenloxP/loxP:Osr1-Cre or PtenloxP/loxP:PB-Cre4 mice were subjected to immunohistochemistry using the Pten and p63 antibodies. Representative images focusing on p63 positive cells (arrows) are shown (Q–T).