Conditional Deletion of the Pten Gene in the Mouse Prostate Induces Prostatic Intraepithelial Neoplasms at Early Ages but a Slow Progression to Prostate Tumors
Figure 2
Development of PIN lesion in PtenloxP/loxP:Osr1-Cre mice.
A–C. H&E staining for 2 to 4 week old PtenloxP/loxP Osr1-Cre mice. Representative images of mouse PIN lesions are shown from different prostate lobes of 2 week old PtenloxP/loxP:Osr1-Cre mice; AP, anterior; D/LP, dorso/lateral, and VP, ventral prostate lobes. Immunohistochemical analyses of these samples show the PIN lesions with concurrent absence of Pten expression (please see Figure S1). The typical high-grade PIN lesions are mainly cribriform or stratified in nature, and often distended the prostate glandular unit profile and/or completely filled the prostate glandular unit lumen. Mitoses were rare. The fibromuscular stroma remained intact, with overall glandular unit profiles being recognizable. D–G. Histological analyses of the prostate tissues isolated from different age groups of PtenloxP/loxP:Osr1-Cre mice. However, the nature and extent of the high grad PIN in PtenloxP/loxP:Osr1-Cre mice did not appear to change with advancing age, as evidence by the similarities of lesions in the 4-week old mice as compared to their 2-, 7-, and 12-month of cohorts.