Retrotransposon Insertion in the T-cell Acute Lymphocytic Leukemia 1 (Tal1) Gene Is Associated with Severe Renal Disease and Patchy Alopecia in Hairpatches (Hpt) Mice
Figure 5
A) Sybr Green qPCR expression analysis of genes in the 6.7 Mb candidate region of Chromosome 4.
Relative gene expression levels of 62 genes from the adult kidneys of Hpt/+ mice (1.5–15 months of age) within the candidate interval are shown (n = 3). Only Tal1 showed a 18-fold increase, whereas no greater than 3-fold change was observed among other genes in the interval. The SybrGreen-TAL1 Probe is located in the non-coding cDNA region of exon 5 (see Fig.7A). B) Tissue-specific expression of Tal1. Relative gene expression levels of Tal1 in Hpt/+ versus +/+ animals using Sybr Green qPCR (n = 3). Tal1 is overexpressed in the kidney, skin, thymus and brain of Hpt/+ mice, while there is no significant expression difference in liver or spleen. As there were no statistically significant differences in the expression values of 4 day, 14 day, 6 month, or 10–15 month Hpt/+ mice tested, expression values for each genotype were pooled from all age groups.