Non-Recessive Bt Toxin Resistance Conferred by an Intracellular Cadherin Mutation in Field-Selected Populations of Cotton Bollworm
Figure 5
Mortality of Sf9 cells exposed to Cry1Ac.
Sf9 cells were transfected with one of four alleles of HaCad (s, r15, r15/s, and s/r15; see Figures 4 and S2 for details) or an empty bacmid (EB), or were not transfected (NT). For cells transfected with alleles of HaCad, LC50 values (95% FL) were significantly higher for alleles with the cytoplasmic domain of r15 (r15: 85 [71–110] and s/r15: 82 [68–100]) than for alleles with the cytoplasmic domain of s (s: 38 [31–46] or r15/s: 38 [31–45]). LC50 values did not differ significantly between Sf9 cells transfected with alleles of HaCad that had the same cytoplasmic domain (r15 and s/r15; s and r15/s).