Functional Cell Types in Taste Buds Have Distinct Longevities
Figure 7
Paired “sister” cells are seen in and around taste buds. A–C,
a pair of cells inside and near the base of a circumvallate taste bud, 1 day after EdU injection. Note that in this single plane confocal micrograph, KCNQ1 staining surrounds each of the two labeled nuclei. D–F, A pair of sister cells with one cell (arrow) still on the outside of the taste bud; the other cell (arrowhead) appears to be transitioning into the KCNQ1-positive area of the taste bud. These images are from a single optical plane micrograph from a mouse 1 day after EdU injection. G-I, An example of an EdU-labeled cell located centrally and away from the basement membrane region. The three panels are consecutive optical sections, each 4–5 µm thick, of a single taste bud.from a mouse 4 h after EdU injection. The appearance of the same taste bud at planes above and below the plane of the labeled cell demonstrates this cell was mid-way through the taste bud in the Z-dimension. Scale bar, 20 µm.