Functional Cell Types in Taste Buds Have Distinct Longevities
Figure 5
Type III cells become EdU labeled only after 3 days and they persist beyond 40 days.
A, B, C, a representative taste bud from a mouse 10 days after i.p. injection, stained for EdU (red), KCNQ1 (grey) and 5HT (green). D, Aggregate data on the incidence of EdU+5HT+ cells in circumvallate taste buds from 35 mice. The solid line is a non-linear decay curve that yields a half-life of 22 days (R2 = 0.54) for Type III cells. The dashed line is a smoothed curve through the mean values at each time point. Scale bar, 20 µm.