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Structure-Activity Relationships of the Antimicrobial Peptide Arasin 1 — And Mode of Action Studies of the N-Terminal, Proline-Rich Region

Figure 5

Effect of arasin 1(1–23), PR-39(1–26) and cecropin P1 on E. coli membrane integrity after 0.5 and 10 min incubation.

Light induction of luciferase expressing E. coli HB101 after AMP treatment at different concentrations is shown. The data presented are relative to the untreated control for the two selected incubation times. The mean of three independent measurements is indicated ± S.D. Note that for the highly membrane active peptide cecropin P1 light emission for the highest concentrations already peaks before the first measurement. MIC against E. coli HB101 is 4, 1 and 1 µM for arasin 1(1–23), PR-39(1–26) and cecropin P1, respectively.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0053326.g005