Genistein Suppresses LPS-Induced Inflammatory Response through Inhibiting NF-κB following AMP Kinase Activation in RAW 264.7 Macrophages
Figure 4
Effect of genistein on NF-κB activation in LPS-treated RAW 264.7 macrophages.
(A) Cells were pretreated with 1, 5,or 10 µM genistein for 1 h, and then incubated with 1 µg/mL LPS for 24 h. Cells were pretreated with 10 µM genistein for 1 h, and then incubated with 1 µg/mL LPS for 0.25, 0.5, 4, or 24 h. (B) Cell lysates were prepared and analyzed for IκB-α, p-IKKα/β, or GAPDH by western blotting. The nuclear fraction was collected for assessment of NF-κB p65 and histones. GAPDH or histone was used as an internal control. Experiments were repeated three times, and representative blots are shown here.