Developmental and Visual Input-Dependent Regulation of the CB1 Cannabinoid Receptor in the Mouse Visual Cortex
Figure 5
Effects of monocular deprivation on CB1 expression.
(A) Representative western blots of CB1 and GAPDH in V1∶2 dMD and 7 dMD indicate monocular deprivation for two days and seven days, respectively. The blots of V1, which is contralateral (cont) or ipsilateral (ipsi) to the deprived eye, are represented with that of the normal animal (NR). (B) Mean and SEM of the blot density of CB1 in MD animals normalized to the mean of normal animals (n = 10 animals each, one-way factorial ANOVA, p>0.05). (C) Representative photographs of CB1 immunoreactivity in V1 of normal and MD animals. Scale, 100 µm. (D) Layer proportion of CB1 immunoreactivity was not significantly different among animal groups (two-way ANOVA, p>0.05). (E) Double immunofluorescent staining of CB1 (magenta) and VGAT (green) in the deep layer of V1 of normal and MD animals. The images of MD animals were obtained in the hemisphere contralateral to the deprived eye. Scale, 3 µm. (F) The CC values of CB1/VGAT in the deep layer of V1, which is contralateral to the deprived eye (n = 3 animals each; n = 386 ROIs (NR), 380 ROIs (2 dMD), 389 ROIs (7 dMD), Bonferroni-corrected Mann-Whitney U-test, **: p<0.0033, ***: p<0.00033).