ADH IB Expression, but Not ADH III, Is Decreased in Human Lung Cancer
Figure 5
GSNOR and ADH gene expression in lung cancer cDNA arrays.
GSNOR (gene name ADH5), ADH IB (ADHIB), ADH II (ADH4), and ADH IV (ADH7) mRNA levels were evaluated by qPCR in human lung cancer cDNA arrays (Origene, #HLRT504, lot #0411, Rockville, MD) and normalized to β-actin levels. Arrays contained 23 matched pairs of normal and cancerous lung tissue. Tumor expression relative to the matched normal sample was calculated using the ΔΔCt method. Relative quantities <1 represent decreased expression in the tumor sample. Lung cancer specimens included Stage IA (n = 4), IB (n = 4), IIA (n = 2), IIB (n = 8), IIIA (n = 3), and IIIB (n = 2) with each sample paired with adjacent normal tissue. No correlation between GSNOR mRNA levels and lung cancer was observed, while expression of ADH IB was strongly reduced in lung cancer samples.