EZH2 Promotes Malignant Behaviors via Cell Cycle Dysregulation and Its mRNA Level Associates with Prognosis of Patient with Non-Small Cell Lung Cancer
Figure 2
The effect of EZH2 down-regulation on the growth of NSCLC cells.
(A, B) Proliferation plot of cells with down-regulated EZH2. A549 or 1299 were transfected with EZH2-targeting siRNA or scrambled siRNA control for 6 hours, then seeded into 96-well plate in triplicate per treatment. The proliferation of the cells was assessed by WST-1 assay at 24 hours interval. (C, D) Distribution of cell population in EZH2 knockdown NSCLC cells. A549 or 1299 cell were transfected with siRNA or scrambled control. The cells were harvested 72 hours later, fixed and stained with propidium iodide. Cellular DNA content were determined by flow cytometry and the distribution of cells is analyzed by FlowJo software.