Recombinant Expression of Margatoxin and Agitoxin-2 in Pichia pastoris: An Efficient Method for Production of KV1.3 Channel Blockers
Figure 6
Amide-proton strips from 3D 15N-edited NOESY spectra of (A) rAgTx2 (G1–K38) and (B) rMgTx (T1–H39).
Sequential αN, βN, and NN connectivities that facilitated sequence-specific resonance assignments are indicated by red, green, and orange lines, respectively. Medium- and long-range dipolar connectivities that facilitated assignment of secondary structure are highlighted and labelled in blue.