Recombinant Expression of Margatoxin and Agitoxin-2 in Pichia pastoris: An Efficient Method for Production of KV1.3 Channel Blockers
Figure 5
1H-15N HSQC spectra of recombinant (A) AgTx2 and (B) MgTx produced in P. pastoris.
Correlation peaks are labelled according to residue type and sequence number. The peaks connected by horizontal lines correspond to the side-chain NH2 groups of Gln and Asn residues. Peaks marked with a red asterisk correspond to residues from the N-terminal His6 tag.