Recombinant Expression of Margatoxin and Agitoxin-2 in Pichia pastoris: An Efficient Method for Production of KV1.3 Channel Blockers
Figure 2
Western blot using anti-His6 primary antibody showing expression and purification of recombinant (A) AgTx2 and (B) MgTx produced in P. pastoris.
The mass (in kDa) of the prestained molecular weight markers (“Stds”) is indicated. Lane 1, culture supernatant after methanol induction of toxin expression; Lane 2, recombinant peptide after purification using nickel affinity chromatography; Lane 3 recombinant peptide after RP-HPLC purification.