Matrix Metalloproteinase 14 in the Zebrafish: An Eye on Retinal and Retinotectal Development
Figure 5
Mmp14a knockdown results in embryos with microphthalmic eyes.
A–D Analysis of eye size (A, C) and total body length (B, D) reveals a dose-dependent decrease in eye size after knockdown of Mmp14a with the ATG- (A, B) or the splice MO (C, D) at 5 dpf. Body length, however, was only decreased after injection of a supramaximal dose of the Mmp14a-ATG MO (B) (n = 55 from 3 independent experiments). E, F Analysis of eye size (E) and total body length (F) (both in µm), in embryos injected with Mmp14a-ATG MO (Mmp14aKD), reveals that knockdown of Mmp14a leads to persistent microphthalmic eyes in embryos/larvae from 3 to 10 dpf while the overall body length is unaffected as compared to control fish (n = 75 from 3 independent experiments). Data are represented as mean ± SEM (*p<0.05, Student's t-test for A–D; multilevel model statistical test (SAS proc mixed) for E–F).