Differential Expression of Alpha 4 Integrins on Effector Memory T Helper Cells during Bordetella Infections. Delayed Responses in Bordetella pertussis
Figure 5
Characterization of lung CD11c+ cells during infection with B. pertussis and B. parapertussis.
(A) Enumeration of lung CD11c+ cells during the early phase of infection with Bordetella sp. Mononuclear cells were isolated from lungs and enriched for DCs (CD11c+) as detailed. After column enrichment, the total number of CD11c+ cells per mouse was estimated using a hemocytometer. (B) CD11c+ enriched lung mononuclear cells were gated on singlets and then on CD11c+ cells (DCs). The frequency of CD11c+ cells expressing maturation markers was assessed at 5 days p.i. (C) and at 25 days p.i. (D), and normalized to the respective cells in uninfected control mice (1.0 by definition). In addition, the frequency of lung CD11c+ cells expressing CCR7 and CXCR4 at 5 days p.i. was measured (E). The bar charts (C, D, E) represent compiled data with error bars showing the SEM of four independent experiments. In each experiment, six mice were used per treatment group. *: p<0.05, and no asterisk indicates p<0.05 as analyzed by a two-tailed student's t-test.