Differential Expression of Alpha 4 Integrins on Effector Memory T Helper Cells during Bordetella Infections. Delayed Responses in Bordetella pertussis
Figure 2
Leukocyte recruitment to the lungs of mice infected with Bordetella strains.
(A) Frozen sections of lungs from BALB/c mice infected with B. pertussis, B. parapertussis, BpΔPTX, or uninfected controls at 5 and 25 days p.i. were H&E stained, and ten independent fields per infection were photographed under 400× magnification. Three independent experiments were performed. (B) Frozen lung sections from mice infected with B. pertussis, B. parapertussis, BpΔPTX, or uninfected controls at 5 and 25 days p.i. were stained sequentially with F4/80 (green) for macrophages, followed by CD45R/B220 (red) for B cells. Alternatively, the slides were sequentially incubated with CD3 (green) for T cells, followed by Gr-1 (red) for neutrophils. Slides were counterstained with DAPI for cell nuclei. Staining with isotype control mAbs revealed no false positive cells (not depicted). Ten separate fields were photographed from each treatment at 100× magnification on a confocal microscope.