Butyrate Produced by Commensal Bacteria Potentiates Phorbol Esters Induced AP-1 Response in Human Intestinal Epithelial Cells
Figure 5
C-fos time course upon PMA induced activation.
Cells were treated with PMA (0,1 µM), butyrate (2 mM), TSA 500 nM, or association of PMA with butyrate or TSA for 0,5, 1 h A), 6 and 24 h B) and total proteins were Western blotted for c-fos. A synergistic effect following PMA/Butyrate co-stimulation is observed after 6 h of stimulation and 24 h. Western blot of GAPDH (lower panel) was shown as loading control. Data are representative of three independent experiments.