AL Amyloid Imaging and Therapy with a Monoclonal Antibody to a Cryptic Epitope on Amyloid Fibrils
Figure 5
Evaluation of size-fractionated Kir BJp preparation as competitors.
(A) Absorbance profile of size fractionated soluble Kir BJp preparation using a Superdex 75 gel filtration column. (B) Fractions, 46, 52, 59 and 66, selected on the basis of the A280 chromatogram trace, were analyzed by SDS-PAGE, and then (C) tested for their ability to inhibit the reaction between 2A4 (200 nM) and Hig L amyloid extract (10 µg/mL) in the standard ELISA. Each fraction was used at the same concentration based on A280 to inhibit binding as described for Fig. 4A.