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Validation of Reference Genes for Real-Time PCR of Reproductive System in the Black Tiger Shrimp

Figure 2

Average expression stability values (M), which is the mean pair-wise variation between an individual gene and all other tested genes, determined by geNorm software.

(A) Average M value of 46 testis samples in P. monodon (TT), (B) Average M value of ovary samples from wild broodstock with different ovarian maturation stages (OV-WB), (C) Average M value of ovary samples from domesticated shrimp with different growth stages (OV-DS) and (D) Average M value of ovary samples from domesticated broodstock before and after eyestalk-ablation (OV-EA) in P. monodon.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0052677.g002