The Drosophila Actin Regulator ENABLED Regulates Cell Shape and Orientation during Gonad Morphogenesis
Figure 5
Live imaging of the SGPs in wild type and ena mutants.
SGPs labeled with PD-six4-egfp::moesin were imaged in wild-type and ena mutant embryos. (A–I) Still images from Movies S1 (A–C) and S2 (D–I) in wild type. At early stage 13, some SGPs had broad migratory protrusions (arrowheads in A), which were lost as gonad coalescence proceeded, and SGPs in the anterior and posterior regions moved to the middle of the gonad (B–C). During coalescence, the SGPs extended protrusions between PGCs (A–I). These protrusions were highly dynamic, and elongated, branched, and connected with each other to encapsulate the PGCs (arrows in D–I). (J–O) Still images from Movie S3 in ena mutants. The ena mutant gonad imaged in (J–O) displayed moderate phenotype similar to the gonad shown in Figure 6C and D. The mutant SGPs normally ensheathed PGCs; however, the anterior and posterior SGPs were unable to move inward resulting in incomplete coalescence. The scale bar in (A) represents 10 µm.