PTH1 Receptor Is Involved in Mediating Cellular Response to Long-Chain Polyunsaturated Fatty Acids
Figure 5
Response of PTH-CC FRET sensor and PM-CC (control FRET sensor) to stimulation by PTH, EPA, EPA and PTH1R inhibitor, [Nle8,18,Tyr34]PTH(3–34), or linoleic acid.
Experiments were done 24 h after transfection of PTH-CC FRET sensor or PM-CC, in HEK293 cells in chambered cover glass at 37°C. FRET ratio was defined as ratio of Citrine emission intensity at 525 nm to Cerulean emission intensity at 475 nm. The FRET response to PTH is from [50].