PTH1 Receptor Is Involved in Mediating Cellular Response to Long-Chain Polyunsaturated Fatty Acids
Figure 2
PTH1R antagonist inhibits ERK1/2 response to PTH(1–34) and fatty acids.
(A) Effect of 1 µM of PTH1R antagonist [Leu11,D-Trp12]PTH-rP(7–34) on pERK stimulated with: 10 nM PTH(1–34), 1 µM EPA and 1 µM DHA in DPBS for 5 minutes in HEK293 cells transfected with PTH1R. (B) Effect of 1 µM of PTH1R antagonist, [Nle8,18,Tyr34]PTH(3–34), on pERK stimulated with 10 nM PTH(1–34), 1 µM EPA, and 1 µM DHA in MC3T3-E1 cells expressing endogenous PTH1R. (C) Dose response to EPA at different concentrations of antagonist [Leu11,D-Trp12]PTH-rP(7–34) in HEK293 cells. (D) Schild plot of [Leu11,D-Trp12]PTH-rP(7–34) antagonism on EPA-stimulated PTH1R in HEK293 cells. Experiments were done 24 h after transfection of HEK293 cells with PTH1R in 12-well plates. Ratio of pERK to ERK was measured using western blots. Data represents the mean of at least four independent experiments.