Synthetic Cationic Peptide IDR-1018 Modulates Human Macrophage Differentiation
Figure 5
IDR-1018 treated monocytes and IDR-1018 differentiated macrophages expressed transcription factors important for the development of alternative (M2) macrophages.
(A) Macrophages were differentiated in the presence of IDR-1018 or the combination of IDR-1018 and M2-inducing factor M-CSF, and RT-qPCR was performed to analyze the expression of different M2 specific transcription factors. (B) Additionally, monocytes were stimulated with IDR-1018 (5 ug/ml) for 4 hours. In both cases, RNA was isolated and the expression of transcription factors was analyzed by RT-qPCR. Mean ± SD results are presented and are representative of 3 biological replicates. *, P<0.05.