Nitric Oxide Participates in Cold-Inhibited Camellia sinensis Pollen Germination and Tube Growth Partly via cGMP In Vitro
Figure 5
Mean relative intensities of fluorescence from NO specific indicator DAF-FM DA in germinated pollen tubes.
Fluorescence images acquired from CLSM as above described (Figure 4) were analyzed and mean relative intensities of fluorescence were calculated using Image J. A–H shows the corresponding mean relative intensities of fluorescence in pollen tubes treated as above (Figure 4). Data are the means of three replicates ± SD. Means with different small letters are significantly different at P<0.05.